Alex F. Metherell, MD, PhD

Research

The Role of Surface and Interface Science in Muscle Contraction
A Testable Model

Abstract: (Download full text)
Few people have paid much attention to the role of surface and interface science of the liquid myoplasm in the contraction process. But in 1970, Albert Szent-Györgyi, in an opening address to a symposium on Muscle, said

“the greatest experience of my scientific life” was the moment I saw “ …motion produced for the first time in vitro by constituents of muscle …” He had earlier succeeded in extracting actomyosin which “ … forms a resilient gel. It is excessively hydrophillic.” He went on to say, “…Under influence of ATP actomyosin loses hydrophillity. The hydrophillic colloid becomes entirely hydrophobic, the most striking change I ever saw.”

Szent-Györgyi recognized in 1940-43 that contraction is triggered by a dramatic change in the surface physics at the fluid filament boundary. Sadly, he abandoned work in muscle when he was unable to reconcile this critical observation with the sliding filament theory.

During contraction the chemical structure of the filaments don’t significantly change, but the chemistry of the myoplasmic fluid changes dramatically. Thus, reinterpreting Szent- Györgyi’s observations, rather than the filaments changing from hydrophilic to hydrophobic, it is the fluid that changes from being proteophilic to becoming proteophobic.

This paper presents an innovative fluid surface energy contraction model. This accounts for force generation, filament sliding, filament shortening, contraction beyond overlap and heat of shortening. The filaments define the fluid/filament boundary. The crossbridges participate in the hydrolysis of the ATP changing the chemistry of the fluid thus changing the surface energy. A surface energy gradient is set up between the overlap and non-overlap region due to the thin film effect in the overlap region which produces force and movement. A meaningful theoretical model must be testable. This paper suggests experiments to confirm this theory.

The full article is found here.

©2005 Alex Metherell